FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8848817
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36750.020.7515281
CTATACT34100.017.0643754
GTTCTAG45900.017.0200231
GTACTAA32800.017.0126111
GTATATA61750.016.5672071
GTCTTAG36750.016.3650261
CCTGTCG26400.016.053535145
GTATTAG35150.015.8752111
CTGTCTA60450.015.850897145
GTTCTAA52100.015.5896331
GTGCTAG33900.015.1803131
GTACTAG20150.015.0772921
GTACAGG61100.014.815421
GTATATG51150.014.66702651
GTATTAA57450.014.3537021
GTCCTAA32600.014.2641531
GTACAAG55300.014.1269271
TAGTACT33600.014.0020374
CTAGACT29700.013.9648174
GGTCTAG28950.013.9208941
Produced by FastQC (version 0.11.5)