FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8875597
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19300.021.496861
CTGTCTA59350.021.149607145
GTTCTAG43300.017.4473511
GTTCTAA50300.017.358361
GTCCTAA33200.016.9730871
GTATATA61950.016.7928921
GTATAGG26250.016.748911
GTCCTAG33850.016.2812941
GTACTAA35500.016.0478551
GTGCTAG34650.015.7266781
GTATTAA57450.015.5213681
GTCTTAG31700.015.4321131
TCTATAC34950.015.3984363
GTATTAG35950.014.6412311
GTATAGA36850.014.11561
TGTACTG65350.013.532565
GCTCTAG40350.013.5050651
GTATATG48350.013.4477741
TATACTG47900.013.4272585
GTCCTAT32500.013.1468981
Produced by FastQC (version 0.11.5)