FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8647519
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA69400.029.070108145
GTATTAG31900.018.6431751
GTACTAG19900.018.0556981
GTCCTAG34550.017.7511481
GGGGGGG452850.017.177998145
GTTCTAG42900.016.8953781
GTGCTAG36950.016.2628481
GTATATA57550.015.393241
CCTATAC25150.015.2727823
GTCCTAA33150.014.5764041
GTACATA44050.014.34476951
TGTACTG62250.014.0329575
TATACTG46700.013.9297055
GTACTAA29800.013.9282921
GTATTAT55600.013.9275451
GTTCTAA46450.013.8703361
GTATTAA56500.013.705691
GTATAGA35700.013.5352331
GTATAAA70500.013.5322791
GTGTTAG38500.013.3553931
Produced by FastQC (version 0.11.5)