FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8647519
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA63700.025.564966145
GTACTAG17750.016.4049511
GTCCTAA32750.016.0798911
CCTGTCG28700.015.938694145
GTCCTAG33250.015.838091
GTGCTAG32600.015.203651
CTATACT35650.015.1100674
GTTCTAG42650.015.1074031
GGTCTAG27600.014.8152971
CCCTTAG36500.014.7673261
GTCTTAG36050.014.6079461
GTATAGG24600.014.6072471
GCTCTAG41700.014.5601391
GTATATG49550.014.5040681
TCTAGAC31600.014.4995273
GTATATA60900.014.3442331
GTACTAA30050.014.0197381
GTTCTAA49200.013.85171
GTATTAA55500.013.8421711
GTATTAG33150.013.8300481
Produced by FastQC (version 0.11.5)