FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8023741
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14550.018.187271
GTACTAG9550.016.1938041
GTACTAA13000.015.8616241
GTATTAA21200.015.2381331
GTCCTAG17000.015.1618481
GTACTAT12700.014.6126781
TCTGTAC33850.014.1125013
GTCTTAG16850.013.6651581
TCTAGAC16200.013.3651363
GCTCTAG22350.013.0701681
GCGATCG3952.4519977E-913.05574837
GTACATA18700.013.0483771
GTGTAGG27950.012.9106241
GTGCTAG19100.012.7751121
CGATCGT4357.6215656E-1012.64588338
GTATTAT20600.012.5122041
CTAGTAC7500.012.3722983
CTGTACT37800.012.3651794
GTACAGG38950.012.0879571
GTCTAGA16400.011.9445771
Produced by FastQC (version 0.11.5)