FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752309
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA19700.015.52487851
GTTCTAG20500.015.4219191
GTCTTAG16950.015.4080721
GTATTAG14750.015.376461
GGTCTAA13300.014.7274531
GTACTAA12800.014.2288681
GTCCTAG18250.013.9339191
GTATTAA17800.013.9000671
GTATAGG12200.013.2386121
GTATTAT20550.013.2105131
GGCTTAG19350.013.141811
GTGCTAA16450.012.9518031
GGTCTAG12650.012.76767351
TCTATAC16100.012.5936083
CTATACT17050.012.4967454
GTACAGG33900.012.4683741
GTCTAGG17150.012.4231581
GTACTAG8050.012.3795991
GCTCTAA18350.012.3598241
GCTCTAG21600.012.250151
Produced by FastQC (version 0.11.5)