FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752309
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82750.10674239120241466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7900.017.8784851
GTTCTAA20550.016.4281331
CTATACT14750.016.3143964
GTATTAA22700.015.9344621
GTGCTAG17000.015.4006361
GTATTAG15300.015.0854181
GTCCTAG18550.015.0423271
TACTAGG9550.014.7803582
GTCTTAG16950.014.6331141
GTACTAA12850.014.2084491
TCTAACC15050.013.9425263
GGTCTAG14150.013.6334431
GTGTTAG18350.013.3289591
GTTCTAG19200.013.0977161
GCTCTAG19300.013.0298531
TCTATAC14300.012.9899023
GTCTATA13450.012.806241
TCTAGAC16450.012.7559283
GTACTGA24100.012.5788111
CCTATAC12950.012.4846453
Produced by FastQC (version 0.11.5)