FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7807414
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6650.018.6140921
GTCCTAA15150.014.9793261
GTCCTAT16500.014.7956951
GTTCTAA20900.014.6421451
GTCCTAG18200.014.5472311
GTATAGG11750.014.339011
GTGCTAG17200.014.1935441
GTCTTAA16750.013.9590251
GCCCTAG18950.013.7900341
GTATATA21050.013.7210761
GTTCTAG19300.013.7181141
GTATTAG14100.013.412341
GTACTAA11750.013.1684791
GTCTTAG15950.013.1501321
GTGCTAA16350.013.0387181
GTATTAA19000.013.0298631
CTGTACT36750.012.0603364
GTGTTAA21400.012.0505941
ATTAGAC12350.011.9629883
GTGTAGG26000.011.9022791
Produced by FastQC (version 0.11.5)