FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7895091
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG18100.016.149121
GTACTAG7150.015.3904911
GTATTAG15200.015.1579261
GTCCTAG18500.015.0564251
GTTCTAG19700.014.3138391
GTATTAA21150.014.3080621
CTATACT15950.013.789374
GTCTTAG14800.013.708781
GTTCTAA21600.013.0547521
CCTATAC12750.012.9380833
GGTCTAG13850.012.9110671
GTCTTAA19300.012.8287321
GTACTAA14250.012.7899711
TCTAGAC16400.012.7827663
TCTATAC13500.012.7284383
CTAGTAC7300.012.7110023
GTACAGG35750.012.6971551
GTATATA21800.012.6194971
GGTCTAA14450.012.61294651
GGGCTAG14800.012.3146671
Produced by FastQC (version 0.11.5)