FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8627069
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA55600.039.560955145
TAGCGCG3550.006628767628.487474145
GGGGGGG509450.017.51844145
GTCCTAA28850.016.8864611
GTATTAG25750.016.4711
GTCCTAG33100.016.2766631
GTATAGG20800.015.4308621
GTATTAA38300.015.2639491
CTATACT25550.014.8056014
TGTACCG13100.014.4382895
GTACCGT10000.014.3294066
CCCCTAG34150.014.2657211
CCTATAC21700.014.2628763
GGTCTAG27800.014.0194061
GTCTTAG32250.013.86214351
CGTGATA10950.006365764413.8534975145
TCTAGAC28600.013.8278963
GTACTAA22900.013.7655241
GCTCTAG40600.013.69339751
CTATACA29100.013.5903044
Produced by FastQC (version 0.11.5)