FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8627069
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA55100.020.891094145
CCTGTCG26000.020.660755145
GCCGTAT16150.020.590698145
GTCCTAG35600.017.249091
GTACTAA22000.016.1734031
GTGCTAG32300.015.6355291
GTATTAA37600.015.2631641
GTGCTAA31350.014.4617881
GTATAGA27550.014.3734131
CCTATAC21850.014.1787783
CTATACT28550.013.8656244
GTATTAG25700.013.8449371
GTACATA32200.013.7235751
GTACTAG14350.013.5975111
GTCTTAA34800.013.5228121
GTCTTAG29450.013.4460961
GTCCTAA28400.013.3369951
GTATATA42250.013.3116461
GTTCTAA37100.013.1485371
GTACTAC20650.013.06201651
Produced by FastQC (version 0.11.5)