FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8697068
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA55450.020.10088145
GCCGTAT17150.019.201853145
GTATTAG25100.016.5046671
GTCCTAG35300.016.462461
GTTCTAA36450.016.4166221
GTCCTAA30650.016.3319021
GTCGCTT16101.5668084E-615.733961145
GTACTAG14700.015.2649471
GTCTTAG29500.015.2132011
GTTCTAG34950.015.1456751
CCTGTCG25603.583409E-1014.84278145
GTACTAA22650.014.7335631
GTATTAA37550.014.2502021
GTCTTAA36700.014.1099181
GCTCTAG40400.014.099441
CTAACCT30750.013.84497454
GTGCTAG32500.013.6318671
CTAGGCC38650.013.3967234
CTAGACA37400.013.2291644
GGTCTAA24600.013.0978781
Produced by FastQC (version 0.11.5)