FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8713306
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA58950.030.035385145
CCTGTCG30950.021.755135145
CGTATCA19850.018.844706145
GCGTTAG6900.00805644718.07089145
GTACTAG17250.016.0340861
GGTCTAA24950.015.9356811
GTATTAA38150.015.557321
GTACTAA25850.015.380861
GTATAAG26600.015.3804421
GTCCTAG33750.015.3659981
GTATTAG26350.015.0890041
CGTATAA9950.003692733415.037887145
GTACATA32950.014.6898011
GTTCTAG36100.014.365721
GCTCTAA35600.013.7581811
GTCCTAA29050.013.6865831
GTGCTAG32550.013.6311291
CTATACT28500.013.5431554
GTGTTAG35050.013.3164691
TACGTGT44950.013.314971145
Produced by FastQC (version 0.11.5)