FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6237074
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG14450.018.9375631
GTATTAG12500.018.5664841
GTTCTAA17200.017.9236491
GCTCTAG17200.016.3125341
GTGCTAG15200.015.4963781
GTACTAG6950.015.4504711
GTATTAA20700.015.0604181
GTACTAA12200.014.7641471
CTATACT14950.014.3382434
GTATTAT18600.014.1535541
GCCCTAG14450.014.1432441
GTCTAGG16000.014.0720781
GTTCTAG17550.013.816111
GTCCTAA13050.013.53706551
GCCTTAG14250.013.1263441
GTCCTAT13250.013.0713071
GTATAGG10650.013.0099381
GTATATG18400.012.8013561
GGTCTAA11100.012.7945721
TGCACTG39750.012.6935625
Produced by FastQC (version 0.11.5)