FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6436213
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7450.020.4064831
GTATTAG12000.019.5794011
GTCTTAG15600.017.2759441
GTATAGG10300.015.4309391
CTATACT14500.014.2984444
GTCCTAG15000.014.28144551
GTATACG3403.916284E-914.2272461
GTATAAT17400.013.9001831
GTATTAA19650.013.5394151
GTATTAC13550.013.5147591
TCTATAC13350.013.4594573
GCTCTAG19050.013.421731
TATACTG17250.013.2208795
GTACTAA12450.013.0436831
CTTATAC13550.013.0057793
CTAGACT12950.012.8078714
GTATAAG14300.012.80594351
GTGCTAG13600.012.7028981
GTCCTAA12800.012.6870211
GTATTAT19650.012.6602331
Produced by FastQC (version 0.11.5)