FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6436213
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14100.016.4316581
GTTCTAA19850.015.8528211
GTATTAA20400.015.7644371
GTATTAT21050.015.1133731
GTGCTAG14450.014.8371181
GTACTAG7850.014.5368391
GTCTTAG15050.014.4753711
TCTAGAC12800.014.3052883
CTAGTAC7100.014.1114073
GTCTTAA18000.014.0241341
GTTCTAT18050.013.9852861
GTCCTAG14950.013.87828351
GTACTAA12750.013.8320231
CTATACT15050.013.7732154
CTTGTAC21800.013.6292723
GTACATA15900.013.4840471
GCCCTAG15400.013.4727481
GTCCTAT14850.013.2731521
GTATATA21750.013.0370781
CGTTCGT2550.00975187712.98841570
Produced by FastQC (version 0.11.5)