FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6379009
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7900.020.1292231
GTATTAG13700.018.420381
GTGCTAG14150.016.8573361
GTCCTAG15050.016.7680531
GTATAGG11450.015.6998011
GTCTATA12300.014.8959111
GGTCTAA10500.014.1571381
GTCCTAA13550.014.0320041
GTACAGG30700.013.9630281
GTATATA21600.013.9239281
GTATTAA20450.013.6926651
GTATAAA26750.013.4402041
GGTCTAG12650.013.39065551
CTATACT15800.013.3470744
GTTCTAA18400.013.33941651
GTATTAT17650.013.3186581
GTACATG25600.013.2337341
GCACTAG11550.013.169431
GCTCTAG17750.013.0488661
TATACTG18900.012.8043155
Produced by FastQC (version 0.11.5)