FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8409764
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9500.017.4417151
GTCTTAG17800.017.0660971
GTATTAG15550.016.2055591
GTCCTAG21550.016.0185891
GTTCTAG22400.015.8730631
GGTCTAG16200.015.7684221
GTACTAA13600.015.7370861
GTATAAG13900.015.6457821
TAGTGCG5700.015.38639957
GTGCTAG20000.015.3614261
TCTAGAC18200.015.3407423
GTATAGG12450.014.9725561
ATAAGCG6150.014.799973556
GTATATA24300.014.3478451
GTCCTAT19650.014.2296431
GTATTAT21150.014.0365251
TCTGTAC34500.013.6878173
CTAGACT17250.013.3800424
CTAGACC16600.013.2813944
TAAGCGT6900.013.21892457
Produced by FastQC (version 0.11.5)