FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190367
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9550.018.0323431
GTCCTAG21900.017.7713261
GTTCTAG22050.015.6198521
GTACTAA14150.015.5779051
CTAGACT16650.015.3084844
GGTCTAG16150.014.7150621
GTCTTAG16650.014.6868831
GTTCTAA20600.014.5457971
GTACAGG36150.014.4817411
GTATAAG17250.014.3756971
TCTAGAC17300.014.1358393
GTGCTAG19800.014.0898161
GTATTAG14750.014.0102121
GTATATA23850.013.7189451
CTAGACC15350.013.4634884
GTACATA21950.013.3373611
CCTAGAC17900.013.2771683
TAGACTG22850.013.2651185
CAATACT20000.013.2609744
GTATAGG13000.013.2468361
Produced by FastQC (version 0.11.5)