FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8259836
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9150.021.4590441
GTACTAA13900.018.5867331
GTATTAG14900.017.8016831
GTATTAA20550.016.2598481
GTCCTAG20000.015.3290991
GTACAGG38250.014.9497271
GTCTTAG17050.014.5467081
GTTCTAG21100.014.529951
GCTCTAG21100.014.529951
GTCTTAA21600.014.193611
GTTCTAA19500.014.1322711
GTATAGG13700.014.0806941
GTGCTAG21450.013.9716781
GTACTAT15150.013.642541
CTATACT15050.013.2758974
GTCTAAG16750.012.9563411
GTCCTAA18350.012.9529771
CTAGTAC9150.012.8002863
TATACTG21200.012.6748155
GGTCTAG16650.012.6203731
Produced by FastQC (version 0.11.5)