FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8259836
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9050.017.5200161
TACGCGA1804.2635384E-617.2239272
GTCCTAG20100.016.8056471
GTTCTAG21550.015.3549781
GTATTAG15450.014.9476021
TAGTACG1851.0367539E-414.8947474
GTCCTAT16350.014.7572541
GTATTAA21550.014.7151871
GCTCTAG24000.014.5055891
GTCTTAG19650.014.3838961
CTAGTAC8350.014.0253983
GTCTTAA21250.013.6252841
GCGATCG3801.3315002E-913.60063837
GCTCTAA23850.013.5851581
GTCCTAA16300.013.5337331
GTGCTAG19450.013.4684991
GTACAGG39950.013.20078851
ATAAGCG6400.013.12682556
GTGCATA19250.013.0712581
TCTGTAC33450.012.9746943
Produced by FastQC (version 0.11.5)