FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13527352
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA37950.022.3567621
GTATTAG44500.021.021541
GTACTAG29250.018.8418081
CGTCTTG57800.017.309517145
GTCCTAG55950.016.5899351
GTCTTAG53000.016.5555761
GCCCTAG57200.016.4809461
GTTCTAG64850.016.4377421
GTATAGG35600.016.2957671
GTGCTAG55600.016.1726681
GTCTAGG51950.016.0526641
TCTAGAC50250.016.0168383
CACGAGC144650.015.988434145
ATAGTAC44300.015.8766153
GGGGGGG6384500.015.837535145
GCCTATG139450.015.648822145
GTATTAA66800.015.41511251
GTATAAG44850.015.3601691
CCCCTAG57350.015.2998351
GTACATA56650.015.2328751
Produced by FastQC (version 0.11.5)