FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13582000
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG26650.022.855641
GTATTAG46750.021.559791
GTCCTAG57200.020.0296061
GTACTAA39950.019.6028081
GTTCTAG62550.018.8960741
CGTCTTG61650.018.816856145
GTCTTAG51450.017.4762251
CTAAGCG16400.017.240594
GTCCTAA47200.017.0526661
GTGCTAG56250.016.8872951
GTACGGA14600.016.8864121
GTATAAG47800.016.6869181
CACGAGC142850.016.444662145
GTATAGG35750.016.4293481
GACCGGC77150.016.164143145
CTATACT47400.016.0598814
CGAATCG10400.016.033299
GCCTATG147300.015.997082145
GTATTAA66150.015.7849781
GTCTAGG53800.015.7693911
Produced by FastQC (version 0.11.5)