FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13582000
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC135650.030.500898145
CGCCGTT23200.026.547722145
CCGTATA20550.024.329523145
GTCCTAG57400.021.8765811
GTACTAA40400.019.2241741
GTACTAG28050.019.1489121
GTATTAG44100.018.927991
CTGCCGC251300.018.367214145
TTACGTT31650.018.31524145
CTATACT48100.018.0806524
GTCCTAA50850.017.7001111
TTATACC92250.017.67309145
GTATTAA66100.017.3500561
GTGCTAG56100.017.2081431
CGTGTAT31950.017.009312145
GTTCTAG65250.016.7973861
GCCGACC55500.016.711418145
GCTCTAG66050.016.3741471
GTGCTAA50650.016.3369391
TAGATCG15550.016.309248145
Produced by FastQC (version 0.11.5)