FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13354664
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT26100.033.05231145
CCGTATA26300.027.839474145
TTACGTT39050.022.091303145
GTACTAG26800.021.3795011
GTACTAA40750.019.4001081
CCGACGC216250.018.83977145
GTCCTAA47850.018.0372491
GTCCTAG53200.017.9956511
GTGCTAG55350.017.5587021
TCTAGAC48500.017.3359743
GCCGACC55200.017.203928145
GTTCTAG61850.017.1205791
GTATTAG43350.017.0653951
GTCTTAG46950.016.9926991
GACGACA59250.016.884418145
GTTCTAA61750.016.795941
CGTGTAT40950.016.640614145
GCTCTAG64450.016.429911
CTATACG10250.016.2646874
GTATTAA66400.016.1658631
Produced by FastQC (version 0.11.5)