FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13365539
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG66450.021.930136145
GTATTAG44800.020.0707451
GTCCTAG58150.019.952171
GTACTAG26900.018.6001591
TACGCGA6303.6379788E-1117.2617992
CGTCTTA43700.017.254093145
GTATTAA63650.017.2027681
GTTCTAG62500.017.0552141
GTGCTAG55300.016.52211
GGTCTAG44350.016.513821
GTATTGG54100.016.4864711
TGCCGTG80900.016.39996145
GCCCTAG54600.016.3354951
CTAGACT46950.016.2137764
GGGGGGG6002150.016.105019145
GCTCTAG66400.015.8350631
GTCTTAG52450.015.62258151
GTTCTAA67100.015.5617981
TCTAGAC49700.015.3166363
GTATAGA43300.015.2395921
Produced by FastQC (version 0.11.5)