FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13365539
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA26150.026.888052145
CGCCGTT27550.026.57413145
TTACGTT39050.020.418835145
CCGACGC229800.020.250896145
GTTCTAG61400.019.9609321
GTATTAG41200.019.0103021
GTCCTAG58200.018.1924971
GTACTAG28800.017.8783861
GTGCTAG57950.017.3949761
GTCTTAG52450.017.2833121
GCCGACC56350.017.237337145
GTATATA81200.017.0584581
GCTCTAG62350.016.981611
TTATACC128850.016.93329145
CTATACT46600.016.6458454
GTACTAA42200.016.4976181
GGTCTAA45300.016.4892711
TCTATAC43600.016.4615143
TCTAGAC50750.016.4279293
GACGACA60850.016.319967145
Produced by FastQC (version 0.11.5)