FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10834144
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13150.016.0023881
GTATTAG22000.015.6804311
GTATAGG17950.014.7981341
GTGTTAG26950.014.59240151
GTCCTAG25600.014.2838931
TATACCG5150.014.03366855
GTGCTAG25350.014.0165121
GTCTTAG26250.013.5359451
GTCTTAA28100.013.5041441
GTACTAA17700.013.4479641
GTTCTAA30700.013.3717811
GTATTAA31400.013.2934091
GTTCTAG27800.013.2776031
TATACTG29150.013.1051975
GTCTAGA23200.012.9363561
TAGTACC15350.012.7823854
CTATACT24100.012.5692984
GCTCTAG31650.012.5344361
GTATAGA23900.012.4131281
GTACAGA47350.012.3853871
Produced by FastQC (version 0.11.5)