FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10471407
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109630.10469462222220949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG23950.016.134861
GTCCTAG28300.015.9712011
GTACTAA18400.014.6261321
GTACTAG11600.014.5744051
GTCCTAA21350.014.3828471
GTTCTAA29450.013.82451
GTTCTAG24400.013.7162261
GTCCTAT21350.013.4132171
GGTCTAG19950.013.3168211
CTAGACC20850.013.2126954
GTATTAG21650.013.06798651
GTGTTAG25300.012.9555491
GTACAGA47100.012.8194651
GCTCTAG30700.012.699681
GTGCTAG26700.012.6639011
TATACTG29750.012.6124595
GTACAGG48050.012.56600951
GTATTAA28850.012.5572981
CTATACT23650.012.5220324
GTGCAGG93300.012.3884191
Produced by FastQC (version 0.11.5)