FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10548373
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11000.020.9571671
GTATTAG19600.018.6080251
GTCCTAG25100.016.0384581
GTGCTAG27350.015.5996491
GTATAGG17850.014.6496021
GTATAAG21100.013.8607561
GTATTAA28550.013.73881151
CCTATAC16950.013.600653
CTATACT23000.013.1650284
GTACAGG48850.013.0303961
GTTCTAG27400.012.9341271
GCTCTAG30100.012.9170231
GTCTTAG24350.012.7172731
TCTATAC22250.012.6805593
GTTCTAA29850.012.6794041
TATACTG28550.012.6548895
GTATATG28050.012.3890781
CTAGTAC11700.012.3514513
GTCCTAA21800.012.3108651
GTATAAA39900.012.2452011
Produced by FastQC (version 0.11.5)