FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10629798
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21850.017.3242231
GTACTAG12100.015.3575141
GTTCTAG29450.014.25565051
GTATAGG19100.014.0531521
GTATTAA31000.013.7648821
GTCCTAA22800.013.4328351
GTGCTAG26800.013.353991
CTAGTAC11350.013.3407273
GTCCTAG24600.013.1493791
GTACTAA18050.012.9641541
GTATACG5700.012.6781821
GTATAAG23250.012.5808071
GTATATA34250.012.5591991
CTAGACC22300.012.345654
GTACATA25850.012.1141611
GTCTAGA21650.012.0800351
GTCCTAT21550.011.816721
GTGTTAG24850.011.7707741
TCTGTAC41400.011.6372513
GTATTAT29400.011.5877831
Produced by FastQC (version 0.11.5)