FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10629798
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21350.017.2574271
GTCCTAG24150.016.539831
GTCTTAG24000.014.9214931
GGTCTAG20950.014.6283781
GTACTAG11300.014.6269231
GTACTAA18600.014.6252851
CTATACT22500.014.0703624
GTGCTAG26300.013.7474991
GTCCTAA23900.013.5431641
GGTCTAA18800.013.18757251
GCTCTAG30750.013.1017981
GTCCTAT21100.013.0556261
GTTCTAG25900.013.0291621
ATTAGAC20150.012.9793463
GTACAGG46500.012.8850611
GTTCTAA30850.012.8360931
GTCTTAA28350.012.7534131
TATACTG28250.012.5460945
TCTATAC19950.012.4194943
GTATAAG20600.012.3695731
Produced by FastQC (version 0.11.5)