FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10986537
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG36450.020.8888421
GGTCTAA37200.019.1031821
GTGCTAG44450.019.0869561
GTCTTAG44450.018.2712751
GTCCTAG46950.017.7617111
GTATTAA59350.017.716171
GTACTAG21250.017.0621451
GTACTAA34850.016.6459961
GTCCTAA36850.015.9393311
GGGGGGG4480050.015.936643145
GTTCTAA54550.015.8188451
GTACAGG80850.015.7853871
GGCTTAG41550.015.7070291
GTATATA60700.015.649671
GTACTAT35700.015.64031
GTATAGG31100.015.6220131
GCTCTAG53850.015.0818591
GTTCTAG52500.015.0553121
CGTCTTA23600.015.052745145
GTACGCG7755.2750693E-1114.9706561
Produced by FastQC (version 0.11.5)