FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10619275
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG44800.022.1730881
GTTCTAG46100.021.70511
GTATTAG37000.020.7724511
CGTCTTG51500.018.72315145
GTACTAA31400.018.7041931
CGTCTTA35000.018.228426145
GTACAGA80600.017.1823251
GTATATG53650.016.7585161
GTATATA61450.016.7552171
TACGCGA3905.622309E-616.7307152
GTCCTAA37450.016.6506141
GTATTAA52750.016.4946231
GTATAGG29500.016.467831
GTTCTAA53350.016.4450241
GTACATA49350.016.3087021
GGGGGGG4183200.016.09365145
GTACTAG22600.016.0415081
GGTCTAG33600.015.7531411
GTGTAGA62300.015.5955381
TCTATAC40450.015.5932743
Produced by FastQC (version 0.11.5)