FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10743652
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT19100.022.77128145
CCGTATA20550.022.575518145
GTACTAG23400.020.7683261
GTATTAG37800.020.7240431
GTTCTAA53150.020.4705981
GTCTTAG41250.020.0458031
GTCCTAG45300.020.0149441
GTATTAA59000.017.826191
GTTCTAG51950.017.5924991
GGTCTAA36650.017.0202921
GCCGTAA19300.016.901482145
GTCCTAT34950.016.810491
GTGTTAG44750.016.6950111
GTCCTAA38600.016.3483721
TTATACC95500.016.167608145
GTCTTAA48200.016.101981
GTACTAA34950.015.9803421
GTATTAT58800.015.9131061
GTGCTAG45700.015.5543711
GTACAGG84100.015.3520561
Produced by FastQC (version 0.11.5)