FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10995324
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14000.020.4556311
GTATTAG20250.017.209141
GTACTAA18900.016.065111
GTATTAA29050.015.4404531
GTCCTAG25700.015.4392981
GTATATA32700.014.983161
GTACTAT21150.014.0297811
GTCTTAG24250.013.9436731
GTGCTAG25100.013.7464051
TATACTG31250.013.5806915
GGTCTAA18450.013.0907491
CTAGACT23300.013.0314734
GTTCTAA32850.013.0241421
GTACAGG48050.012.925341
TCTATAC21750.012.84962753
GTCTAGC15600.012.8282161
CTATACT24250.012.8055314
GTCCTAA21300.012.7970641
GTTCTAG28600.012.7880011
GCCCTAG28200.012.7246861
Produced by FastQC (version 0.11.5)