FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11026045
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13100.018.1736431
GTACTAA18300.017.3460641
GTATTAG20200.016.2269341
CTATACT24700.014.9473724
GTCTTAG21650.014.3432911
GTTCTAG28950.014.3020051
GTCCTAG27600.014.2514811
GTATTAA30350.014.0970161
GTGCTAG25150.013.8563111
TCTATAC22300.013.6157663
GTATATA33300.013.262641
GTTCTAA31950.012.9590921
GTATATG31550.012.7953071
TCTGTAC48450.012.6050253
GTCCTAA22850.012.5330311
GTACATG43000.012.517581
GGTCTAA20450.012.4854051
GCCTTAG25150.012.3472081
GTCTTAA28250.012.3357961
GTCTAGA24000.012.220021
Produced by FastQC (version 0.11.5)