FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11026045
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19900.017.901591
GTACTAG12100.016.5786861
GTGCTAG25800.015.4164931
GTATTAA31950.014.93881
GTCCTAA23450.014.4540881
GTCTTAG24550.014.3699791
GTACTAA18700.014.24152851
GCCCTAG26500.013.8346271
GTTCTAG31800.013.5953491
GTACATA27750.013.5853551
GTATAGG18200.013.4925621
GTACTAT21550.013.3210451
GGTCTAG19800.013.2756521
GTACAGG48050.013.0284471
TAGTACT19150.012.9786924
GCCTTAG23500.012.9515661
GTCCTAG28150.012.9008511
CTATACC20400.012.8602874
GTATATA33050.012.6625561
GTATTAT28500.012.4997061
Produced by FastQC (version 0.11.5)