FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11312416
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21550.016.0111081
TACGCGA2603.3036486E-815.9250352
GTCCTAG28650.015.8971021
GTATATA34350.014.8663251
TCTATAC21700.014.7875333
CTATACT23200.014.4265984
GTATTAA31850.014.1915731
GTATAGG17350.013.92089751
GTGCTAG25800.013.9085641
GTACTAG13450.013.8528811
GTCTTAG23800.013.772581
GGTCTAA20300.013.7675821
GTACTAA21000.013.4729661
GTTCTAG31700.012.7348921
CCTATAC17600.012.5469973
GTATATG32350.012.47901251
TAGACTG30550.012.4240625
GTACAGG51350.012.2964381
GCTCTAG33250.012.24500751
GTGTATA26500.012.2391321
Produced by FastQC (version 0.11.5)