FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3879610
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG6400.018.341271
TATACTG10000.017.2628175
GTACTAG4600.017.2623731
GTCCTAG9250.016.7958221
GTATATA12200.016.4134041
GTATAAT10600.016.2852571
TTATACT13000.015.6693274
GTCTATA7700.014.7963191
GTCTTAG9350.014.7699451
TGTACCG3554.638423E-1014.5882975
GTACTAA6200.014.478121
GTATTAA9800.014.4440271
GTACAGG17850.013.9259481
ATTATAC11550.013.4515463
GTCTTAC11600.013.393221
GTACTAC5200.013.2787481
GTTCTAG10250.013.1362451
GTCCTAA7900.013.1106621
TCTAGAC9300.012.993523
GCTAGAC5850.012.9839993
Produced by FastQC (version 0.11.5)