FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3879610
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7100.019.9871731
GTCCTAG9850.017.5695111
GTCCTAA7700.017.530721
TCTATAC7400.017.280733
GTCTTAG9000.016.9213921
CTAGCGC1250.001918765516.58953
CTATACT8650.016.3722234
TTGCGCG1503.5177937E-416.10602212
GTGCTAG10600.015.3468021
GTATTAA12050.015.2235081
GTGCTAA9350.014.8072451
GGTCTAG6400.014.6019111
CTAGACT7900.014.4286864
TTAGACA12000.014.3922494
GTATTAT12150.014.2435961
GCCCTAG9000.014.2293531
GTTCTAG11600.014.0238021
CGATCGT1500.006504687513.808938
GTACTAT5800.013.7254231
CTGTACT18700.013.6687894
Produced by FastQC (version 0.11.5)