FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830831
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG6450.020.3367671
GTCCTAG8900.017.453421
GTCTAGG9300.017.0739081
GTACAGG17000.015.6350711
GTATAGG6650.015.5724751
GTACTAA6450.015.52016451
GTGCGTA1808.055328E-515.3417731
CTAGACT8400.015.2047914
TATACTG10900.015.20122055
GGTCTAA5950.015.0839271
GTTCTAG11500.015.0082551
GTACTAG3959.094947E-1214.8562741
GTATTAA11000.014.74902251
GTACAAG12900.014.7173971
GTCCTAA8200.014.3127511
GTACTAC5950.013.9236251
TCAGTAC10450.013.873663
GTATAAG7750.013.8075961
GTGTACG4001.891749E-1013.8075951
TCTAGAC8550.013.7268493
Produced by FastQC (version 0.11.5)