FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12538595
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27200.017.8402211
GTACTAG15800.015.9006831
GTCTTAG29600.014.9985061
GTCCTAG32400.014.6583161
GTGCTAG30150.014.2683151
GTTCTAG40450.014.1234091
GTATTAA37050.014.1190471
GTTCTAA34950.013.8842341
GTACTAA24350.013.4268661
GTCCTAA28050.013.1280721
GCTCTAG35500.012.8935731
GTATATA51050.012.6739531
TCTGTAC52350.012.5569453
GTATATG39750.012.5539641
GTGCTAA30800.012.5146091
TGTACTG49950.012.471765
GTATAAG27200.012.3995861
GTATAGA30600.012.3714691
CTATACT28500.012.3179454
CTGTACT62450.012.2900974
Produced by FastQC (version 0.11.5)