FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12538595
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16100.017.3679281
GTCCTAG33300.016.5868761
GTCTTAG28750.016.3301411
GTTCTAG36950.016.0695191
GTGCTAG32300.015.3903631
GTGCTAA31550.015.2091271
GTACTAA24350.015.0278131
GTATTAG24750.014.9244191
GTATTAA38000.014.807881
GTCCTAA26800.013.7828121
GTACAGG54250.013.426771
TATACTG38600.013.2889525
CTATACT30200.013.0012114
TCTATAC27050.012.8679823
GCTCTAG34650.012.8521371
GTATATA48200.012.8202011
GTATAGG20650.012.7052261
GTCTATA25550.012.7006451
GCCCTAG32800.012.6297941
GTACCGT8000.012.4755946
Produced by FastQC (version 0.11.5)