FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12716833
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17650.016.7698611
GTATTAG27650.016.5551151
GTCCTAG32350.016.4904731
CTATACT30650.015.0483654
GTCTTAG30100.014.4072061
GTATTAA38250.014.3067641
GTACTAA24450.014.2173361
GTTCTAG38550.014.1061481
TATACTG38250.013.9481625
GTCCTAA29650.013.581161
GTATATA47450.013.4187251
TCTAGAC29450.013.3237353
GTATAGG18600.013.13775351
TCTGTAC50800.012.9412463
GTGCTAG34600.012.9313231
GTATAAG25700.012.7223171
GTTCTAA37100.012.6165531
GTACTAT22400.012.445511
GTACAGG57600.012.3686871
TCTATAC27600.012.346183
Produced by FastQC (version 0.11.5)