FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12612787
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27100.017.7947941
GTGCTAG29300.016.105981
GTCCTAG34500.015.5754171
GTTCTAG37450.014.8084051
GTACTAG15150.014.3239281
GTACTAA22950.014.1084531
GTCTTAG27950.013.8029031
GTCCTAA28700.013.8022581
CTAGACT30850.013.6106494
GTATTAA36450.013.5136521
GGTCTAG26300.013.4901161
GTATATA48950.012.8071621
GGTCTAA25000.012.6759941
GTCTTAA36500.012.5514231
GTATTAT38200.012.5338831
GTATAGG19400.012.4288381
GTATAGA29950.012.4211311
TGTACTG47400.012.2708795
GTGCTAA31350.012.1960621
GTGTTAG35100.011.9725571
Produced by FastQC (version 0.11.5)