FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12575125
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28250.016.689591
GTATTAA37850.015.5479741
GTCCTAG33600.015.4661161
GTACTAG13600.015.1829421
GTACTAA25050.014.83746051
CGAATCG4251.8189894E-1214.5758559
GTGCTAG34000.014.57562351
GTATAGG22000.013.9222971
GCTCTAG35950.013.59355451
GTACAGG57050.013.5728331
GTTCTAA36200.013.4046081
CTATACT28850.013.3607124
GTATATA46200.013.184841
GTCTTAG29800.013.0498571
GTTCTAG36450.013.0294211
GTCTTAA35600.012.9538361
GTCCTAA27200.012.9055011
GTATAGA29600.012.9054991
GTACTAT24000.012.6187111
GTGTTAG35100.012.4520311
Produced by FastQC (version 0.11.5)