FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15157700
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18750.017.0897241
GTCTTAG33600.016.7148191
GTATTAA43100.015.8285541
CGATCGT6050.015.37883238
GTCCTAG38200.015.1530231
GTATTAG28350.014.8272331
GTATAGG23500.014.8083561
GTACTAA28650.014.7922351
CTATACT33150.014.3432934
GTGCTAG35700.014.2839011
GTATATA49950.014.2097051
GTCCTAA31200.014.1354191
GCGATCG5900.014.01678537
GTACAGG66400.013.3876691
GTCTTAA41500.013.3669141
TCTATAC30950.013.1363493
GCTCTAG42650.013.0872781
TCTGTAC63350.012.8900633
GTTCTAG44200.012.784241
GTACTAT26750.012.7516021
Produced by FastQC (version 0.11.5)