FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15097446
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192420.12745202069277148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29000.017.7576831
GTCCTAG39650.017.2591721
GTACTAG18050.016.850141
GTGCTAG38200.016.01431
GTACTAA25350.015.951661
GTATTAA42950.015.933091
GTCTTAG35900.014.9222821
GTTCTAG42200.014.5782561
TCTATAC30800.014.5662433
CTATACT36200.013.9067224
GTTCTAA45500.013.9007331
GCCCTAG37300.013.806241
GCCTTAG37250.013.7319891
GTCCTAA30350.013.5514661
GTATAGG24500.013.5426351
GTACAGG65550.013.4451451
GGTCTAG29600.013.4277781
GTATTAT44300.013.1850261
TACGCGA2901.9376112E-613.1009932
GTATATA51450.013.0992751
Produced by FastQC (version 0.11.5)