FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15319902
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG41500.015.3619851
GTCCTAG40200.014.9158131
GTATTAA43100.014.8716581
GTATTAG28800.014.8372341
TCTATAC31700.014.3479143
CTATACT32750.014.203544
GTATAGG24950.014.0881291
GTACTAG19850.014.06203651
GTCTTAA39950.014.06031
GTCTTAG35650.013.9195991
GCTCTAG42700.013.3968881
GTGCTAG36400.013.3487761
GTCTATA27700.013.1871161
GTATATG42850.013.0283051
TCTGTAC63650.012.9923263
GTACTAA27100.012.9704371
GGTCTAG28750.012.9452261
GTATATA52200.012.7412051
ATAGTAC26800.012.728423
CCTATAC25800.012.6875573
Produced by FastQC (version 0.11.5)